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Sabato, 14 Settembre 2019

I. Ricci et al.

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Performance and trends of the results of the Interlaboratory Trials held between 2002-2017 for the serological tests employed for the diagnosis of Equine Infectious Anemia

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  • Riferimento: Large Animal Review 2019; 25: 141-145
  • Abstract: The aim of this paper is to analyse the trend of the Interlaboratory Trails (IT) for serological techniques used in the diagnosis of Equine Infectious Anaemia (EIA), organized by the National Reference Centre for EIA between 2002-2017. The participation of numerous national laboratories and the long time interval considered gives greater value to the data obtained. The increase in the samples to be tested as prescribed by the legislative provisions, and the requirement to participate with a satisfactory outcome for accreditation purposes has made this tool important for verifying the technical and diagnostic competence of the laboratories. The performances obtained by the laboratories demonstrate the efficiency and the reliability over time of the diagnostic system, which plays a central role in the surveillance activities. Overall, although the whole diagnostic system has an acceptable sensitivity, the ELISA presented higher concordance levels and better reproducibility than the agar gel immunodiffusion (AGID). The implementation, since 2007, of the regular serological testing for EIA requested the use of highly sensitive techniques, such as the ELISA; this assay indeed is capable of detecting about 20% more positives samples than AGID and can more easily detect weak positive sera that, in an advanced stage of eradication, constitutes the majority of the positive samples and are difficult to diagnose with the previous test, since those with a strongly positive reaction have been already recruited and removed through previous surveillance activities. The high number of laboratories that immediately participated in the IT for ELISA, on its introduction, reflects its diagnostic value owing to its high sensitivity, underlined by the reduced percentage of errors obtained for the positive sera. This makes it an effective method, in support of the eradication of infection to be applied during the screening phase of the national surveillance program, to recruit sera not detected in AGID.
  • Note: //
  • Specie: animali da reddito
  • Anno: 2019
  • Rivista: Large Animals Review
Letto 64 volte Ultima modifica il Sabato, 14 Settembre 2019

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